Journal article
An Integrated Coproscopic and Molecular Method Provides Insights into the Epidemiology of Zoonotic Intestinal Helminths of Dogs across Cambodia
PA Zendejas-Heredia, V Colella, LG Huggins, R Schaper, B Schunack, RJ Traub
Transboundary and Emerging Diseases | WILEY | Published : 2023
DOI: 10.1155/2023/2001871
Abstract
Introduction. In Cambodia, a limited number of focal surveys targeting dogs in rural communities have reported intestinal helminths of potential zoonotic risk as highly endemic. In this study, we investigated the prevalence, diversity, distribution, and risk predictors associated with zoonotic intestinal helminths infecting pet, community, and pagoda dogs across rural and urban settings in Cambodia through an integrated coproscopic and molecular approach. Methods. Faecal samples, demographic, and clinical data were collected from 457 dogs across Cambodia. Faeces were screened for gastroenteric parasites using sodium nitrate faecal floatation (1.30 SpGr) and multiplex TaqMan-based quantitativ..
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Awarded by Australian Research Council
Funding Acknowledgements
This research was funded by an Australian Research Council Linkage grant number LP170100187 with Bayer Animal Health-an Elanco Animal Health company as industry partners. P. A. Zendejas-Heredia is a recipient of a Research Training Programme Scholarship (University of Melbourne). V. Colella is a University of Melbourne McKenzie Research Fellow and a Soulsby Fellow. R. Traub is an Australian Research Council Future Fellow. The authors are grateful to the staff at Cambodia Mine Action Centre and Animal Mama Veterinary Hospital for their assistance with fieldwork and sampling logistics within Cambodia. We are grateful to Prof. Robin Gasser and A/Prof. Neil Young for their assistance and advice on the selection of gene targets for conventional PCR. We also thank the 2022 Annual Conference of the Australian Society for Parasitology for giving us the opportunity to present these results.